13787   Rpl10     Status: ES cell colonies screened / QC no positives

GeneRpl10
ChromosomeX
Deletion Start74,271,005
Deletion End74,273,112
Deletion Size2,108
Genome Build38
MGI IDMGI:105943
NCBI Gene ID110954
BAC ID426P6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,271,18974,271,207AGGCTGTGGGTGGCGTTAC
TUP74,271,21874,271,244AGCGCTTGGGTAGAACTAGCATGTCCG
TUR74,271,24974,271,268GCGACGCAGAGGATTGGATG
TDF74,272,14774,272,170CCCTGTTTCACTGCTGTTATTTCC
TDP74,272,17374,272,196ATTCAGCACTGGAGGCTGCCCGTA
TDR74,272,21674,272,239CCATCCTTGCCACAACTCTTTACC

PCR Primers

PrimerSequence
SUATGTGTCGAAATCCCATTCG
SDGCTCCAGTAGAAATGGGCTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR80 bp++-
TDFTDR93 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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