13793 Rpl3l Status: ES cell colonies screened / QC no positives
| Gene | Rpl3l |
| Chromosome | 17 |
| Deletion Start | 24,733,312 |
| Deletion End | 24,736,057 |
| Deletion Size | 2,746 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,733,561 | 24,733,581 | CCGCCAGCCAATCCCATTTAC |
| TUP | 24,733,587 | 24,733,610 | TGACCAGTCGCTGGCATACCAAGA |
| TUR | 24,733,642 | 24,733,660 | GCCGATGCAGGCCACTTTG |
| TDF | 24,735,353 | 24,735,372 | TCCCACTACAGCCTCCTAAC |
| TDP | 24,735,373 | 24,735,393 | TTGGCTGCCCTGTTCCTGGCT |
| TDR | 24,735,396 | 24,735,417 | CCTACAGGGAGACAGACCACAC |
PCR Primers
| Primer | Sequence |
| SU | ACTATGCTGTGACCTAACTG |
| SD | GCTAAGCTCCAGTACAGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
