13802 Rps2 Status: ES cell colonies screened / QC no positives
| Gene | Rps2 |
| Chromosome | 17 |
| Deletion Start | 24,720,279 |
| Deletion End | 24,721,984 |
| Deletion Size | 1,706 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,720,777 | 24,720,799 | CTCACTCTGCATGGTTACACAAG |
| TUP | 24,720,804 | 24,720,828 | TACCCGTGTGACTTTCGTACGGAGA |
| TUR | 24,720,833 | 24,720,855 | GTCCTGAAGGAACTCGTTTTCTC |
| TDF | 24,721,509 | 24,721,528 | GCTTAGGTTGCATGGCAGAC |
| TDP | 24,721,533 | 24,721,558 | CCCAAGGTGGCTTCTCTGTTCCTAGG |
| TDR | 24,721,586 | 24,721,606 | GGGTTGGTGGCTTCATTAGAG |
PCR Primers
| Primer | Sequence |
| SU | TTTATACGTCCTTCCGGCTG |
| SD | CTTTCCTCTCCAGCTTTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
