| Gene | Saa3 |
| Chromosome | 7 |
| Deletion Start | 46,715,097 |
| Deletion End | 46,712,041 |
| Deletion Size | 3,057 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,715,076 | 46,715,097 | AAGCCTTCCATTGCCATCATTC |
| TUP | 46,715,047 | 46,715,073 | TGCATCTTGATCCTGGGAGTTGACAGC |
| TUR | 46,715,004 | 46,715,026 | GCCTACCTTGACCAGCTTCTTTC |
| TDF | 46,712,594 | 46,712,612 | AGCCTGGGCTGCTAAAGTC |
| TDP | 46,712,567 | 46,712,593 | ATCAGGTAACACGGGTCTGGGTGAACA |
| TDR | 46,712,546 | 46,712,566 | CTGTCCAAGGCAGTCAATCTC |
PCR Primers
| Primer | Sequence |
| SU | CCACATAGCCCTCTGGAAAC |
| SD | TGCTTGGCAGTCATGCTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13807A-B7 | VGB6 | C57BL/6NTac | |
| 13807A-C10 | VGB6 | C57BL/6NTac | |
| 13807A-E6 | VGB6 | C57BL/6NTac | |
| 13807A-C1 | VGB6 | C57BL/6NTac | |
| 13807A-A1 | VGB6 | C57BL/6NTac | |
