13809 Sall2 Status: ES cell colonies screened / QC no positives
| Gene | Sall2 |
| Chromosome | 14 |
| Deletion Start | 52,328,172 |
| Deletion End | 52,312,648 |
| Deletion Size | 15,525 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,328,023 | 52,328,042 | TCAAAGCCTGCATGGTTCAG |
| TUP | 52,328,000 | 52,328,021 | CCAGGGCAATGGAGGGCACCTC |
| TUR | 52,327,923 | 52,327,943 | AGCCCTCTCCTCATCCTCAAG |
| TDF | 52,312,875 | 52,312,895 | GACCGTGCTACCCTCAAGAAG |
| TDP | 52,312,848 | 52,312,872 | CATGCTGTTGGCTCACCACCAGGTA |
| TDR | 52,312,790 | 52,312,810 | AGCCAGGGACCAAGGAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | TTAGAACAGCAAGCCTTGGG |
| SD | AGCTTCTCAAGAAGGTCCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
