13812 Sap30l Status: ES cell colonies screened / QC no positives
| Gene | Sap30l |
| Chromosome | 11 |
| Deletion Start | 57,802,095 |
| Deletion End | 57,810,119 |
| Deletion Size | 8,025 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,802,587 | 57,802,608 | CGGGAGGATCTTCTCCAACTTC |
| TUP | 57,802,610 | 57,802,632 | TCCTCCCTCCAGGGACCTTGTCG |
| TUR | 57,802,641 | 57,802,658 | GGCCACCTTGCTAGTGTC |
| TDF | 57,809,811 | 57,809,829 | CGGCTCCTTGGCAAGTCTG |
| TDP | 57,809,830 | 57,809,850 | AGTGCCAGGCCATCGCTGCTG |
| TDR | 57,809,858 | 57,809,879 | CGCTGCAAAGAGGGAAGAGAAG |
PCR Primers
| Primer | Sequence |
| SU | TACGACCATTGAGCGCACAG |
| SD | ACATCCCTCTGTTCCCAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
