13816 Scoc Status: ES cell colonies screened / QC no positives
| Gene | Scoc |
| Chromosome | 8 |
| Deletion Start | 83,458,336 |
| Deletion End | 83,435,896 |
| Deletion Size | 22,441 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,457,447 | 83,457,469 | GCCAGACAATGAACACAAACCAC |
| TUP | 83,457,420 | 83,457,443 | TCTGGCTGCCGGCTATAAACATGC |
| TUR | 83,457,361 | 83,457,384 | CACCTTGTACCTTTCCCTCGATAG |
| TDF | 83,436,148 | 83,436,170 | GGGTATCACACATGCTAGGCAAG |
| TDP | 83,436,121 | 83,436,144 | TGTAGCACTGAGTCACCCCACAGC |
| TDR | 83,436,098 | 83,436,120 | TCCATGTTACAAATGCAGACCAG |
PCR Primers
| Primer | Sequence |
| SU | ACCATTGTCTGCCAGGCTAC |
| SD | AGCTGTGCATTCAGTACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
