13817 Scrib Status: ES cell colonies screened / QC no positives
| Gene | Scrib |
| Chromosome | 15 |
| Deletion Start | 76,069,384 |
| Deletion End | 76,047,294 |
| Deletion Size | 22,091 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,068,795 | 76,068,817 | TGAGTGGGACTCTCCTAAGATAC |
| TUP | 76,068,759 | 76,068,786 | TCAGATCTATTCTGTCATCGCTCAGCGG |
| TUR | 76,068,727 | 76,068,749 | GGAAGGAACACAGACAGTGAAAG |
| TDF | 76,047,640 | 76,047,659 | TGGAGGAGCTGAGGACTTTG |
| TDP | 76,047,611 | 76,047,637 | AGCATCTCCCAGTCCTGGTGAGTAGGC |
| TDR | 76,047,590 | 76,047,608 | GGGCACTCAACCCAGACAG |
PCR Primers
| Primer | Sequence |
| SU | AGCGCCTAAGAAAGCGCTG |
| SD | GCCCAGTGGAAGGTTCTAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
