| Gene | Sct |
| Chromosome | 7 |
| Deletion Start | 141,279,095 |
| Deletion End | 141,278,378 |
| Deletion Size | 718 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,278,902 | 141,278,924 | CACTCAGACGGAATGTTCACCAG |
| TUP | 141,278,879 | 141,278,899 | AGCTCAGCCGCTTGCAGGACA |
| TUR | 141,278,784 | 141,278,804 | CCTGTCCCATACTAGCCAGTC |
| TDF | 141,278,709 | 141,278,727 | GTCAGCGAGCAGGACACAG |
| TDP | 141,278,679 | 141,278,705 | ATATCCCAGAGAACAGCCTGGCCCGGT |
| TDR | 141,278,640 | 141,278,658 | TCGACCACAGCAAGCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGCTCCAAGATGAGTTGAGG |
| SD | GCCAGGATGTACTCAGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13818A-C2 | VGB6 | C57BL/6NTac | |
| 13818A-D9 | VGB6 | C57BL/6NTac | |
| 13818A-E3 | VGB6 | C57BL/6NTac | |
| 13818A-D6 | VGB6 | C57BL/6NTac | |
| 13818A-E8 | VGB6 | C57BL/6NTac | |
| 13818A-G3 | VGB6 | C57BL/6NTac | |
