13826 Srsf6 Status: ES cell colonies screened / QC no positives
| Gene | Srsf6 |
| Chromosome | 2 |
| Deletion Start | 162,931,674 |
| Deletion End | 162,934,758 |
| Deletion Size | 3,085 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 162,932,385 | 162,932,405 | TGGAAGGAGTGGCTTCAGTTG |
| TUP | 162,932,408 | 162,932,434 | CCTCTTTGGCACTGACTTGGGTTCTGC |
| TUR | 162,932,435 | 162,932,455 | AGCCAGCCCTCGCTTTCAATG |
| TDF | 162,934,584 | 162,934,607 | GAGAACGGCAAAGGAGACATAAAG |
| TDP | 162,934,608 | 162,934,631 | TCAAAGTCCAGATCCCGGAGCCAG |
| TDR | 162,934,650 | 162,934,669 | TTTGAGGGTGGAGCAGGTAG |
PCR Primers
| Primer | Sequence |
| SU | TAGGCACGACTCAAAAGGTC |
| SD | AAGCCTGCAGTATCCAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
