13827 Srsf9 Status: ES cell colonies screened / QC no positives
| Gene | Srsf9 |
| Chromosome | 5 |
| Deletion Start | 115,327,312 |
| Deletion End | 115,332,727 |
| Deletion Size | 5,416 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,327,353 | 115,327,371 | GCGCATCTACGTGGGCAAC |
| TUP | 115,327,380 | 115,327,401 | CGACGTGCGCGAGAAGGACCTC |
| TUR | 115,327,470 | 115,327,487 | AAGCGCACGAAGGCGAAG |
| TDF | 115,332,086 | 115,332,109 | GTTGTGGTCTTCTCTTCCATTCTC |
| TDP | 115,332,112 | 115,332,136 | CCTAGGACTTCCTCCATCAGGCAGC |
| TDR | 115,332,148 | 115,332,170 | CAGCTTCTCGCATGTGATCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCGCTCGCATCTCTAG |
| SD | GACAACTGCACGCCAATTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
