13828 Sh3bp5l Status: ES cell colonies screened / QC no positives
| Gene | Sh3bp5l |
| Chromosome | 11 |
| Deletion Start | 58,331,684 |
| Deletion End | 58,346,414 |
| Deletion Size | 14,731 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,331,730 | 58,331,752 | AACTTCGGTCAGAGGTGGTAGAG |
| TUP | 58,331,762 | 58,331,781 | CCTAGGAGCCCGGTCGCAGA |
| TUR | 58,331,787 | 58,331,808 | CTGTTGCTTCCACTTCCTCCAG |
| TDF | 58,345,973 | 58,345,991 | AGGCCAAGACCCGCTACTC |
| TDP | 58,345,994 | 58,346,016 | TGGCCCTGCGTAATCTGGAGCAG |
| TDR | 58,346,019 | 58,346,038 | TGGCATGAATCTGCTCACTG |
PCR Primers
| Primer | Sequence |
| SU | TTTGCTCCTTGAGTTGGAGC |
| SD | AAAGCCTGTCCAACCACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
