13835 Slc25a39 Status: ES cell colonies screened / QC no positives
| Gene | Slc25a39 |
| Chromosome | 11 |
| Deletion Start | 102,406,636 |
| Deletion End | 102,403,392 |
| Deletion Size | 3,245 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,405,722 | 102,405,739 | GCTGTGCCACCTGGTTTC |
| TUP | 102,405,695 | 102,405,716 | CCCACACGGTTCACTGGCACCT |
| TUR | 102,405,661 | 102,405,682 | TCCCTCAGAGACACATTGATTC |
| TDF | 102,403,697 | 102,403,715 | AGTGAAGCCGCCCAGAGTG |
| TDP | 102,403,673 | 102,403,695 | ACTCCACCTGGCTCCTGCTTCGC |
| TDR | 102,403,620 | 102,403,642 | CACAGAGACAGACCTGCAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTTTGATCTTTCGGCCTCTG |
| SD | CCCAAGACACTACAGCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
