13836 Slc30a1 Status: ES cell colonies screened / QC no positives
| Gene | Slc30a1 |
| Chromosome | 1 |
| Deletion Start | 191,907,010 |
| Deletion End | 191,909,831 |
| Deletion Size | 2,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 191,907,199 | 191,907,216 | CACGCCACGCAGAAGAAC |
| TUP | 191,907,217 | 191,907,237 | ACGTTCGGCTGGATCCGCGCT |
| TUR | 191,907,315 | 191,907,336 | CATCTCATGCGGCTCGATGAAG |
| TDF | 191,909,495 | 191,909,514 | AGCGTCGGCTCTAAGTCAAG |
| TDP | 191,909,515 | 191,909,535 | TGTGCTCCCGTGTGAGCTCGC |
| TDR | 191,909,548 | 191,909,565 | CACTGCTTCAGGGCACAC |
PCR Primers
| Primer | Sequence |
| SU | TTCCGAGTGCAAAGCAAAGTT |
| SD | CCCTCAAACATCAAAGGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
