| Gene | Slc7a6os |
| Chromosome | 8 |
| Deletion Start | 106,210,910 |
| Deletion End | 106,200,951 |
| Deletion Size | 9,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,210,674 | 106,210,695 | TTTGTGGGAGCGATCTTTGAAC |
| TUP | 106,210,649 | 106,210,671 | TGCCCGTGTCGCAGTCATCTCTT |
| TUR | 106,210,606 | 106,210,624 | CGCACAAGCTGCTGGATTG |
| TDF | 106,201,866 | 106,201,888 | TGAGAGGCAGAGGGAGATAGATG |
| TDP | 106,201,838 | 106,201,862 | ATCTCAGCTCAAGGACAGCCTGGTC |
| TDR | 106,201,800 | 106,201,821 | CTTTGTGTATCCCTGGGTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCTGACACAAGTCCTGCG |
| SD | AAGACATGACCGCCCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13848A-D8 | VGB6 | C57BL/6NTac | |
| 13848A-C8 | VGB6 | C57BL/6NTac | |
| 13848A-B7 | VGB6 | C57BL/6NTac | |
| 13848A-B12 | VGB6 | C57BL/6NTac | |
| 13848A-A1 | VGB6 | C57BL/6NTac | |
| 13848A-C6 | VGB6 | C57BL/6NTac | |
