| Gene | Smcr8 |
| Chromosome | 11 |
| Deletion Start | 60,778,031 |
| Deletion End | 60,780,464 |
| Deletion Size | 2,434 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,778,787 | 60,778,807 | CGCTCATACCCTCGTCAGAAG |
| TUP | 60,778,809 | 60,778,833 | CGAAGATCCCTGACTTGCAGCCTGG |
| TUR | 60,778,879 | 60,778,900 | AGCGGACTCCTCAGGATTAGAG |
| TDF | 60,780,223 | 60,780,243 | GGTCCTGGGCGAAGATGAAAC |
| TDP | 60,780,244 | 60,780,269 | CATTGTCAGGAAGCTGGTGACTGCAC |
| TDR | 60,780,286 | 60,780,308 | GCTTGGCATAGCAGCCATAGTTG |
PCR Primers
| Primer | Sequence |
| SU | ACCTAGCTTCAGGAACCTTG |
| SD | CGAAGACTGCTGTGTTTACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13849B-A5 | VGB6 | C57BL/6NTac | |
| 13849B-C1 | VGB6 | C57BL/6NTac | |
| 13849B-H2 | VGB6 | C57BL/6NTac | |
| 13849B-H8 | VGB6 | C57BL/6NTac | |
| 13849B-H9 | VGB6 | C57BL/6NTac | |
