| Gene | Smgc |
| Chromosome | 15 |
| Deletion Start | 91,838,372 |
| Deletion End | 91,860,775 |
| Deletion Size | 22,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,838,466 | 91,838,487 | CACGTTTAGTGTTCTGCACTTC |
| TUP | 91,838,488 | 91,838,507 | TTCCTTGTGCTGCAAAGTAG |
| TUR | 91,838,508 | 91,838,529 | TGCCGTATCCCTGCATACTATC |
| TDF | 91,860,262 | 91,860,284 | TTTGTCAGGTGACTCCAATGAAG |
| TDP | 91,860,286 | 91,860,308 | AAGAGGGCCAAGCTCCTCAGCAG |
| TDR | 91,860,320 | 91,860,339 | TTCCCACGGTCTCCTGAATC |
PCR Primers
| Primer | Sequence |
| SU | AGGCATATTCTGTGGTTGGC |
| SD | TGGATGACCAGTCACAAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13850A-A9 | VGB6 | C57BL/6NTac | |
| 13850A-B6 | VGB6 | C57BL/6NTac | |
