13855 Rdbp Status: ES cell colonies screened / QC no positives
| Gene | Rdbp |
| Chromosome | 17 |
| Deletion Start | 34,856,162 |
| Deletion End | 34,850,922 |
| Deletion Size | 5,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,851,198 | 34,851,217 | CCACCCAGAGCCTTGTGAAC |
| TUP | 34,851,220 | 34,851,247 | TCAAATGCCCAAGTAAATGTGCTCCTCC |
| TUR | 34,851,249 | 34,851,270 | AAGGGAGGGAGGTAGAGCAGAC |
| TDF | 34,855,324 | 34,855,344 | GCCTCTGAGCTGGAGTGTTAC |
| TDP | 34,855,346 | 34,855,371 | TGAACTTGGTATTGTGGGTCTGGACC |
| TDR | 34,855,379 | 34,855,399 | ATGTTTCCATGCCTGGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCTCTGGTTGCCCAGATAG |
| SD | CCACATCAACCTCTTCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
