13860 Spsb3 Status: ES cell colonies screened / QC no positives
| Gene | Spsb3 |
| Chromosome | 17 |
| Deletion Start | 24,887,499 |
| Deletion End | 24,891,797 |
| Deletion Size | 4,299 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,887,517 | 24,887,535 | GTCATGGGTGTCTGGGTTG |
| TUP | 24,887,538 | 24,887,559 | TTTCACACCATCGCCCTGGGCT |
| TUR | 24,887,586 | 24,887,604 | TCCCACAAGCTCGTACCTG |
| TDF | 24,891,418 | 24,891,437 | TGCCAAGAGCAGCATGAAAG |
| TDP | 24,891,438 | 24,891,455 | TCATCCGCTCCTGCGCCA |
| TDR | 24,891,475 | 24,891,493 | CAAGCGGTAGCAGCACAGG |
PCR Primers
| Primer | Sequence |
| SU | ACAAAGACAGTCCACTCGGG |
| SD | AGCTCTCTCAAAGCCGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
