13863 Ssna1 Status: Cancelled
| Gene | Ssna1 |
| Chromosome | 2 |
| Deletion Start | 25,272,346 |
| Deletion End | 25,271,455 |
| Deletion Size | 892 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,272,142 | 25,272,161 | CCACCCACAGCTCTGTCTTC |
| TUP | 25,272,119 | 25,272,140 | CACCCACCCTAGGCATCGAGGA |
| TUR | 25,272,099 | 25,272,118 | CCTCTCGCTTCTGACACAAC |
| TDF | 25,271,845 | 25,271,864 | TGGTTCAGAGGCTGTAGGTG |
| TDP | 25,271,817 | 25,271,842 | ACAGTGGGCTGTCTGTGGGTTCCAAA |
| TDR | 25,271,764 | 25,271,784 | TGGGTTAATTCAGGCCCACAC |
PCR Primers
| Primer | Sequence |
| SU | ACCGGTGCTCACAGTATTCC |
| SD | CTTCAACATGTCTGGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 63 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
