13874 Crisp2 Status: ES cell colonies screened / QC no positives
| Gene | Crisp2 |
| Chromosome | 17 |
| Deletion Start | 40,790,652 |
| Deletion End | 40,765,204 |
| Deletion Size | 25,449 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,790,486 | 40,790,506 | TCCAGAGTATCCTGGCAGGTG |
| TUP | 40,790,457 | 40,790,479 | TGTCTCGGGTGGCTCAAAGCACT |
| TUR | 40,790,404 | 40,790,427 | GCCATGAGTGATGGAGCAAATAAC |
| TDF | 40,766,044 | 40,766,066 | GGGTGTATTACTGCAACATGAGC |
| TDP | 40,766,012 | 40,766,038 | AAACAGCCAGTGTTTGAGTGCTGGGTA |
| TDR | 40,765,967 | 40,765,988 | GACCAGGAATCAAACTGCACTC |
PCR Primers
| Primer | Sequence |
| SU | AGTCTCTGCATGTGCAAGTC |
| SD | CTGGCAGGCGCTATAAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
