13882 Thap1 Status: Cancelled
| Gene | Thap1 |
| Chromosome | 8 |
| Deletion Start | 26,158,446 |
| Deletion End | 26,162,796 |
| Deletion Size | 4,351 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,158,952 | 26,158,970 | GATGCCGGGAAGGAAGCAG |
| TUP | 26,158,979 | 26,159,002 | ACCGGGATGCCAGGGAGGACTCTT |
| TUR | 26,159,005 | 26,159,025 | TTGGTTCTTTGACGCTAGGAC |
| TDF | 26,162,614 | 26,162,634 | GAGCAGCAGGTGGAGAAACTC |
| TDP | 26,162,638 | 26,162,660 | AAGAAGCTCAAGACGGCCCAGCA |
| TDR | 26,162,694 | 26,162,712 | GTGGACGACTTCCTTGAGC |
PCR Primers
| Primer | Sequence |
| SU | CTGGGAACGTTACCTTCAGC |
| SD | CTGAAAGGCTCTGTCCTATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
