13900 Rhoq Status: ES cell colonies screened / QC no positives
| Gene | Rhoq |
| Chromosome | 17 |
| Deletion Start | 86,997,155 |
| Deletion End | 86,963,609 |
| Deletion Size | 33,547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,963,714 | 86,963,734 | GAGTACGTGCCCACTGTCTTC |
| TUP | 86,963,737 | 86,963,759 | CCACTACGCAGGTACGCCTCTGC |
| TUR | 86,963,794 | 86,963,814 | TCAGCCAAAGTTGCTCCAAAG |
| TDF | 86,996,292 | 86,996,315 | CAACCCAGTAGAACACGTTAAGAC |
| TDP | 86,996,319 | 86,996,346 | ACCGTGCTCTTAGGAGACCGTAAAGGAG |
| TDR | 86,996,362 | 86,996,381 | CACATTCCCTTCTGGCACTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTATGATCCGGCGGATCC |
| SD | CATCTCCTGTACACTCGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
