| Gene | Hrasls5 |
| Chromosome | 19 |
| Deletion Start | 7,639,124 |
| Deletion End | 7,612,614 |
| Deletion Size | 26,511 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,613,311 | 7,613,331 | CCCTTCCCTCCTGAACTAGAC |
| TUP | 7,613,333 | 7,613,356 | CAGCGAGTTCTCAAGCGCTGTTGG |
| TUR | 7,613,361 | 7,613,380 | TGAGTTGCTTCGGCAAGAAC |
| TDF | 7,638,220 | 7,638,242 | CCTTCAAATTCCTGGGTGAATCC |
| TDP | 7,638,243 | 7,638,265 | TCCCATCTTGGCTCTGTCCCAGA |
| TDR | 7,638,271 | 7,638,291 | TGGGACCATTGGTAGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TTTGTGTCTCATGCTAGCGG |
| SD | GAATGTTTCCGATGGTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13914B-B5 | VGB6 | C57BL/6NTac | |
| 13914B-C6 | VGB6 | C57BL/6NTac | |
| 13914B-G12 | VGB6 | C57BL/6NTac | |
| 13914B-H1 | VGB6 | C57BL/6NTac | |
