| Gene | Triap1 |
| Chromosome | 5 |
| Deletion Start | 115,341,274 |
| Deletion End | 115,342,802 |
| Deletion Size | 1,529 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,341,285 | 115,341,306 | GGAGGCTTGCACTGACATGAAG |
| TUP | 115,341,307 | 115,341,331 | CGCGAGTACGACCAGTGCTTCAACC |
| TUR | 115,341,393 | 115,341,411 | GCACTGCTGGTAGCGTTTG |
| TDF | 115,342,255 | 115,342,277 | GGGATGGTAACTCAAGGTCACAG |
| TDP | 115,342,278 | 115,342,303 | AGCCGATTCGGAGTGAACACTGTCAC |
| TDR | 115,342,313 | 115,342,334 | AAGGTCAGTTCAGGTGCCTATC |
PCR Primers
| Primer | Sequence |
| SU | ATGGCTTTCTCTAGCCGGTC |
| SD | TTCAGAGCATCCCTGAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13915B-G6 | VGB6 | C57BL/6NTac | |
| 13915A-G4 | VGB6 | C57BL/6NTac | |
