| Gene | Uqcrq |
| Chromosome | 11 |
| Deletion Start | 53,430,686 |
| Deletion End | 53,429,005 |
| Deletion Size | 1,682 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,430,402 | 53,430,420 | ACTCCAACGGCTCCCTCTG |
| TUP | 53,430,376 | 53,430,401 | AGGTCATCGCTGGCCACTATCTGTGA |
| TUR | 53,430,333 | 53,430,352 | GGCCGCACATCTAACACAAG |
| TDF | 53,429,528 | 53,429,550 | GGACAGGCAGAATTGGTTGAAAG |
| TDP | 53,429,501 | 53,429,527 | TCAGAAAGGAGGACATGGACTGACAGA |
| TDR | 53,429,465 | 53,429,487 | TGGACAGACAGACCTCAAACAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCTTGGTACTGGTCCTTTC |
| SD | TCCACCTGACACCCTGATTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13937A-B8 | VGB6 | C57BL/6NTac | |
| 13937A-C2 | VGB6 | C57BL/6NTac | |
| 13937A-F10 | VGB6 | C57BL/6NTac | |
| 13937A-F7 | VGB6 | C57BL/6NTac | |
| 13937A-E3 | VGB6 | C57BL/6NTac | |
