| Gene | Wdr55 |
| Chromosome | 18 |
| Deletion Start | 36,760,290 |
| Deletion End | 36,763,425 |
| Deletion Size | 3,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,760,547 | 36,760,567 | CGGGAAGGCATCTTATGAGAC |
| TUP | 36,760,568 | 36,760,588 | TCAGACGGCCCGAATCGTTCA |
| TUR | 36,760,589 | 36,760,608 | GCACACTTGCCGAATGTTAC |
| TDF | 36,762,664 | 36,762,682 | TGGCTGCCTTGGTGTCTTC |
| TDP | 36,762,685 | 36,762,712 | CATCAAGAGACGCCGGTTTGAGTTACTC |
| TDR | 36,762,734 | 36,762,753 | ATGAGGGCGACGGAGGTTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTGCAGTGCCTTTCTTCC |
| SD | TTTGGAGAACTGGCTGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13943A-A4 | VGB6 | C57BL/6NTac | |
| 13943A-C12 | VGB6 | C57BL/6NTac | |
| 13943A-E1 | VGB6 | C57BL/6NTac | |
