| Gene | Ypel1 |
| Chromosome | 16 |
| Deletion Start | 17,077,941 |
| Deletion End | 17,084,689 |
| Deletion Size | 6,749 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,077,979 | 17,078,000 | GCCACACTGTCACCGAACTTAC |
| TUP | 17,078,001 | 17,078,023 | AGCTGCATCCACTGCAGAGCCCA |
| TUR | 17,078,051 | 17,078,072 | TGAGTCCAGATCACTTGCCTTG |
| TDF | 17,083,885 | 17,083,905 | GTGTCTTCTGGCCTCCATGAG |
| TDP | 17,083,909 | 17,083,931 | TGCACATGCGTGATGCACAGACA |
| TDR | 17,083,943 | 17,083,962 | TGGGCGTATCCGTGTATGTC |
PCR Primers
| Primer | Sequence |
| SU | TGTAACTTTTAGGCCTACTCC |
| SD | CGCCTTCTACATCAGGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13947A-A11 | VGB6 | C57BL/6NTac | |
| 13947A-G1 | VGB6 | C57BL/6NTac | |
| 13947A-D5 | VGB6 | C57BL/6NTac | |
| 13947A-E10 | VGB6 | C57BL/6NTac | |
| 13947A-C4 | VGB6 | C57BL/6NTac | |
| 13947A-H8 | VGB6 | C57BL/6NTac | |
