13956 Zfp157 Status: ES cell colonies screened / QC no positives
| Gene | Zfp157 |
| Chromosome | 5 |
| Deletion Start | 138,441,630 |
| Deletion End | 138,457,322 |
| Deletion Size | 15,693 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,441,966 | 138,441,988 | CCGTCTCTTCATCTTGCATCTCC |
| TUP | 138,441,989 | 138,442,016 | ACGTTTGTCCCTGGCTTCTCAAGTCACA |
| TUR | 138,442,045 | 138,442,063 | TGCTCACGGGCTTCCATAG |
| TDF | 138,457,238 | 138,457,261 | TCAGGTGAGACACGCTAATAACAG |
| TDP | 138,457,262 | 138,457,289 | AGAAGGCTAGAAAGCATGCAGTCAACCA |
| TDR | 138,457,294 | 138,457,316 | AAGGCATCCTCTGATGTCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTTACCCATGAAAGCAAGGC |
| SD | ACGGAACATAAGCAAGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
