| Gene | Zfp334 |
| Chromosome | 2 |
| Deletion Start | 165,386,937 |
| Deletion End | 165,379,987 |
| Deletion Size | 6,951 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,386,750 | 165,386,770 | AAGGCATTGCCAGATGTGCTG |
| TUP | 165,386,724 | 165,386,748 | AGCACGCCTTTAATCCCAGCGCTTG |
| TUR | 165,386,680 | 165,386,699 | ACCAGGCTGGCTTCTCACTC |
| TDF | 165,380,278 | 165,380,296 | GCCACAAGTCGGCCTTCAC |
| TDP | 165,380,254 | 165,380,277 | CGTGCACAAGAGGATCCACACCGG |
| TDR | 165,380,211 | 165,380,231 | GCGGTAGCTCTTGCCACATTC |
PCR Primers
| Primer | Sequence |
| SU | GCCTGCTCTTTCAGGCTTC |
| SD | TGCAGATGAAGGGATTAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13960A-H3 | VGB6 | C57BL/6NTac | |
| 13960A-G3 | VGB6 | C57BL/6NTac | |
| 13960A-E11 | VGB6 | C57BL/6NTac | |
| 13960A-C5 | VGB6 | C57BL/6NTac | |
| 13960A-A4 | VGB6 | C57BL/6NTac | |
