| Gene | Zfp513 |
| Chromosome | 5 |
| Deletion Start | 31,201,604 |
| Deletion End | 31,199,291 |
| Deletion Size | 2,314 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,201,488 | 31,201,510 | ACCCGAACACCACTTTCTACAAC |
| TUP | 31,201,459 | 31,201,485 | AGAACTCCCTAAAGGTCCCTCAGCAGC |
| TUR | 31,201,428 | 31,201,447 | CCGAACCCGAACTTCACCTC |
| TDF | 31,199,805 | 31,199,822 | GGCTCGGCACATGAAGAC |
| TDP | 31,199,780 | 31,199,803 | CACAGTGGTGAGAAACCCTTCCGC |
| TDR | 31,199,747 | 31,199,767 | CAGATGAGCAGAGGCGTATGG |
PCR Primers
| Primer | Sequence |
| SU | TAGAGTGGGCTCAGGTCAGG |
| SD | CTTTGGTTCCCTCTCACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13962A-A5 | VGB6 | C57BL/6NTac | |
| 13962A-D2 | VGB6 | C57BL/6NTac | |
| 13962A-D5 | VGB6 | C57BL/6NTac | |
| 13962A-F7 | VGB6 | C57BL/6NTac | |
| 13962A-E12 | VGB6 | C57BL/6NTac | |
| 13962A-G10 | VGB6 | C57BL/6NTac | |
