13966 Zfp68 Status: ES cell colonies screened / QC no positives
| Gene | Zfp68 |
| Chromosome | 5 |
| Deletion Start | 138,616,487 |
| Deletion End | 138,606,168 |
| Deletion Size | 10,320 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,616,263 | 138,616,285 | TCAACTCCTGTTGGAACTCTTTG |
| TUP | 138,616,238 | 138,616,262 | TGCCGTTAGTTCCCTACTGACCGTA |
| TUR | 138,616,201 | 138,616,221 | TGTACCCTGTGCCCATTGATG |
| TDF | 138,607,054 | 138,607,078 | TGGAAAACCAGCTTCAATAGACATC |
| TDP | 138,607,028 | 138,607,051 | AGCACTCACACTGGTGAGAAGCCC |
| TDR | 138,606,973 | 138,606,992 | GCTGAGTGAGGTGTGACTTC |
PCR Primers
| Primer | Sequence |
| SU | CACAGTACCTGTTAGGCTGGC |
| SD | TCCTCACTTTCGCTGGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
