13969 Zfp93 Status: ES cell colonies screened / QC no positives
| Gene | Zfp93 |
| Chromosome | 7 |
| Deletion Start | 24,271,659 |
| Deletion End | 24,276,539 |
| Deletion Size | 4,881 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,272,426 | 24,272,450 | TGGAAAATGATTAAAAATGGCAGTT |
| TUP | 24,272,453 | 24,272,477 | TCTGAAACTTTCACTTACTTAATTA |
| TUR | 24,272,499 | 24,272,527 | TGACTATAATGTACTGTCCATTTACAAAC |
| TDF | 24,276,230 | 24,276,251 | AGCAAGACCTCAAACCTCCAGG |
| TDP | 24,276,252 | 24,276,274 | CCCACCAGAGGGTTCACACAGGG |
| TDR | 24,276,415 | 24,276,434 | GGACCCTCTGGTGAGAACTC |
PCR Primers
| Primer | Sequence |
| SU | TGAAGGTAAGAGTGCCCAGC |
| SD | AAGCTCCTATGAAGTCTCCTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 205 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
