| Gene | Znrd1 |
| Chromosome | 17 |
| Deletion Start | 36,958,281 |
| Deletion End | 36,954,469 |
| Deletion Size | 3,813 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,957,680 | 36,957,704 | GTCTCCATAACCCTTCTGTCTCTTC |
| TUP | 36,957,656 | 36,957,678 | TAGATTGACAGGCGCTGCCCTCG |
| TUR | 36,957,608 | 36,957,625 | GCTGAGCGCATCTGTCTG |
| TDF | 36,955,056 | 36,955,079 | GGACTGGAGTTAAAGATGGTTGTG |
| TDP | 36,955,030 | 36,955,053 | CCACCGTGTGGTTGCTAGGGATCA |
| TDR | 36,954,972 | 36,954,991 | GCTGGAGATTTGGCTCACTG |
PCR Primers
| Primer | Sequence |
| SU | TAGTACCATACTGCGTGCCG |
| SD | AAACCTCATTCCAGACTCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13973A-A12 | VGB6 | C57BL/6NTac | |
| 13973A-C1 | VGB6 | C57BL/6NTac | |
| 13973A-G11 | VGB6 | C57BL/6NTac | |
| 13973A-D12 | VGB6 | C57BL/6NTac | |
