13976 Prss42 Status: ES cell colonies screened / QC no positives
| Gene | Prss42 |
| Chromosome | 9 |
| Deletion Start | 110,798,201 |
| Deletion End | 110,803,218 |
| Deletion Size | 5,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,798,450 | 110,798,474 | TCAAGGTCCACACTAAGAGCTAATG |
| TUP | 110,798,478 | 110,798,500 | CCCTGAGAGTCACGGGTGTCGCT |
| TUR | 110,798,513 | 110,798,534 | GCAAGGCAACCGAACTCTACAC |
| TDF | 110,802,491 | 110,802,511 | GAACTGGAGTGGCAGGTGATG |
| TDP | 110,802,513 | 110,802,536 | TGGGTCACCATGTGGGTGTTCTCA |
| TDR | 110,802,538 | 110,802,556 | GCAGAGGACGCTGGATTAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGAGGTGCCTCTTTGGAG |
| SD | TGATGACATCCTTGCTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
