13979 Aldh1a1 Status: ES cell colonies screened / QC no positives
| Gene | Aldh1a1 |
| Chromosome | 19 |
| Deletion Start | 20,630,846 |
| Deletion End | 20,602,015 |
| Deletion Size | 28,832 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,602,566 | 20,602,585 | AAGGCTGGAGGACATGACTC |
| TUP | 20,602,586 | 20,602,612 | TGAGCATCCCATGGAAACTATTGCCAA |
| TUR | 20,602,675 | 20,602,696 | GTGTTCTCCAACAGCAACAAAC |
| TDF | 20,630,662 | 20,630,682 | GCAGTGGACATTGCTGTTGAG |
| TDP | 20,630,689 | 20,630,717 | CACCATGGAGTGTTTTATCATCAAGGCCA |
| TDR | 20,630,723 | 20,630,742 | AATCCGGGATGCTGCGACAC |
PCR Primers
| Primer | Sequence |
| SU | CACCCTTAGCACAGGTTTGG |
| SD | GCTTCCTAAACTATATCTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
