13980 Fam108a Status: ES cell colonies screened / QC no positives
| Gene | Fam108a |
| Chromosome | 10 |
| Deletion Start | 80,586,843 |
| Deletion End | 80,583,827 |
| Deletion Size | 3,017 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,586,259 | 80,586,277 | AGGGCCAGGCAGGTGTATG |
| TUP | 80,586,221 | 80,586,249 | TGACACAGGCCTTTGTCTTATTACAGGTC |
| TUR | 80,586,180 | 80,586,200 | GAGACCCATCCTGTCCAGTTG |
| TDF | 80,584,524 | 80,584,542 | GGCAGCACTGGGAAGAGAC |
| TDP | 80,584,501 | 80,584,523 | AGCCCTCTGCTCGTGGGTCAGAA |
| TDR | 80,584,444 | 80,584,462 | GTGCCTGACTGTGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AGGATCAAGATAGACCGTGG |
| SD | AATCTTCGCATCCAGAGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
