13994 0610007P08Rik Status: ES cell colonies screened / QC no positives
| Gene | 0610007P08Rik |
| Chromosome | 13 |
| Deletion Start | 63,916,786 |
| Deletion End | 63,950,163 |
| Deletion Size | 33,378 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,917,464 | 63,917,485 | GCAGCAAGTTGGCTTACAGATG |
| TUP | 63,917,489 | 63,917,515 | TCAACCTTCAAGCCCTGACAGAACAGA |
| TUR | 63,917,520 | 63,917,539 | GGGCACAGAACACTCAGTTG |
| TDF | 63,949,268 | 63,949,291 | TCAGCTCTAATCAGTCAGTCAATC |
| TDP | 63,949,295 | 63,949,320 | TCAGACACAGCTTCTTTCCAGAGGCA |
| TDR | 63,949,375 | 63,949,397 | GAATACTGAACTCAGCACCTTGC |
PCR Primers
| Primer | Sequence |
| SU | CTCCTAGACGACAAGGCCAC |
| SD | TAAGGCAGAAGATTCAATTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
