13995   0610010K14Rik     Status: ES cell colonies screened / QC positives

Gene0610010K14Rik
Chromosome11
Deletion Start70,051,369
Deletion End70,048,965
Deletion Size2,405
Genome Build37
MGI IDMGI:1915609
NCBI Gene ID104457
BAC ID183L4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGCTCAGTGTAGGTGGTAATTGTTG
TUPTGGATGACAGGTAAAGAGACTACCGAGGC
TURGGGTAGGTGGCAGACATTC
TDFGGTGTCCTCTCACCTCCAAAC
TDPCCTCCACCCAGCAGCTCCAGTG
TDRAGCAACTCCACAGCCCTCAC

PCR Primers

PrimerSequence
SUCTTGCTGTCTGTGCTTTCAG
SDAATATTGGCTGTGCTGCTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13995A-H10VGB6C57BL/6NTac
13995A-B4VGB6C57BL/6NTac
13995A-H3VGB6C57BL/6NTac
13995A-E11VGB6C57BL/6NTac
13995A-E3VGB6C57BL/6NTac
13995A-D8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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