14002   1110036O03Rik     Status: ES cell colonies screened / QC positives

Gene1110036O03Rik
Chromosome11
Deletion Start100,276,403
Deletion End100,270,439
Deletion Size5,965
Genome Build37
MGI IDMGI:1913430
NCBI Gene ID66180
BAC ID359A23
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCGAGAAATACAGCTTCCGAGGATTC
TUPTCCGGAGGACCTGATGCCACTG
TURCGCCTTCGTATTGCTCCAGAG
TDFGGAACTGGAGGAGACAGAATCAC
TDPTCCCAGAGCCTGAAGAACCTCTATCTGA
TDRCATAGTCGCCCTCGCCTTC

PCR Primers

PrimerSequence
SUGCCAGTGGTGGTGTAAGCATTG
SDCATAGGGTACACCAACTGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14002A-B10VGB6C57BL/6NTac
14002A-H4VGB6C57BL/6NTac
14002A-C9VGB6C57BL/6NTac
14002A-E2VGB6C57BL/6NTac
14002A-D6VGB6C57BL/6NTac
14002A-C7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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