| Gene | 1110058L19Rik |
| Chromosome | 1 |
| Deletion Start | 24,005,597 |
| Deletion End | 23,996,250 |
| Deletion Size | 9,348 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,005,257 | 24,005,275 | GCAGAGGCATGAGGATCTC |
| TUP | 24,005,229 | 24,005,256 | TGAGTTCAAGGTCAGCCTGGTTCAAACG |
| TUR | 24,005,197 | 24,005,218 | TTGTTCTTTGCTCTACGTGTGC |
| TDF | 23,996,450 | 23,996,469 | GATGACCCAGGCACTCACAC |
| TDP | 23,996,419 | 23,996,447 | AGATCATGCAGTCCTGTCCTCAACATTGA |
| TDR | 23,996,393 | 23,996,415 | AGCAGAGACGGCGAGAAATGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCATCGGAATAGCTAAGAGC |
| SD | CTCTCATCCCAGACTCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14004A-D8 | VGB6 | C57BL/6NTac | |
| 14004A-E5 | VGB6 | C57BL/6NTac | |
| 14004A-F4 | VGB6 | C57BL/6NTac | |
| 14004A-G9 | VGB6 | C57BL/6NTac | |
| 14004A-G12 | VGB6 | C57BL/6NTac | |
