14005 1110059E24Rik Status: ES cell colonies screened / QC no positives
| Gene | 1110059E24Rik |
| Chromosome | 19 |
| Deletion Start | 21,652,705 |
| Deletion End | 21,619,114 |
| Deletion Size | 33,592 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,652,438 | 21,652,456 | ATTCTTCCCACGCCCTTTC |
| TUP | 21,652,415 | 21,652,437 | TTTGGCTTGGCTCTGTTCCGTGA |
| TUR | 21,652,358 | 21,652,377 | AAGCTGCCCTATCCAGTCTC |
| TDF | 21,619,404 | 21,619,427 | GCATGTTCTCCATAGTTTGCATTG |
| TDP | 21,619,379 | 21,619,398 | TCTACCATGTAGGTCCAGGG |
| TDR | 21,619,357 | 21,619,378 | GCCTGAGGCCCTGAATTTAATC |
PCR Primers
| Primer | Sequence |
| SU | AGATTCGAAGAAAGCCGGAC |
| SD | TCCAGTTGTCTTCAACTTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
