14009 1700009P17Rik Status: ES cell colonies screened / QC no positives
| Gene | 1700009P17Rik |
| Chromosome | 1 |
| Deletion Start | 171,123,170 |
| Deletion End | 171,126,845 |
| Deletion Size | 3,676 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 171,123,236 | 171,123,253 | CCAGATGCTGCCCTTGAC |
| TUP | 171,123,254 | 171,123,275 | TGCCCGCTCCCATCAGACTCTG |
| TUR | 171,123,320 | 171,123,341 | CCCAGATCCTCTTTGCTTGTTC |
| TDF | 171,125,975 | 171,125,994 | CCCTGCCAAGCAGACACTAC |
| TDP | 171,125,995 | 171,126,018 | CAGCCGTGAAGACCTGGTGAGCAA |
| TDR | 171,126,051 | 171,126,070 | TTTGCCAGGTGCCCATGAAG |
PCR Primers
| Primer | Sequence |
| SU | CAGTGTGTGATGGATGAGGG |
| SD | GAATGCGTGTAATAAAGGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
