| Gene | 1700016D06Rik |
| Chromosome | 8 |
| Deletion Start | 11,678,666 |
| Deletion End | 11,655,541 |
| Deletion Size | 23,126 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,678,548 | 11,678,567 | CCCAGGAGACTGGCAGATAG |
| TUP | 11,678,517 | 11,678,543 | CTATAGGCACGATGAATCAGGCAGGCA |
| TUR | 11,678,485 | 11,678,508 | GAGCCCTTTCAAACAATAGCAGAG |
| TDF | 11,655,631 | 11,655,653 | CCAGCAAAGTAGAAGCAGTCAAC |
| TDP | 11,655,595 | 11,655,622 | AAGCCAATCCAGCCCTAAGTACTAGGCC |
| TDR | 11,655,572 | 11,655,590 | GCAGTGGCTTGTCCCTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGGAAGGAGATATGATGGGC |
| SD | ACAGACAAGGGAAGGTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14011A-D3 | VGB6 | C57BL/6NTac | |
| 14011A-F12 | VGB6 | C57BL/6NTac | |
| 14011A-E8 | VGB6 | C57BL/6NTac | |
| 14011A-F4 | VGB6 | C57BL/6NTac | |
