14012 1700019N12Rik Status: ES cell colonies screened / QC positives
| Gene | 1700019N12Rik |
| Chromosome | 19 |
| Deletion Start | 6,999,857 |
| Deletion End | 6,997,044 |
| Deletion Size | 2,814 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,999,576 | 6,999,594 | GGTGCCCAAACACGCAAAC |
| TUP | 6,999,553 | 6,999,575 | CATCGCCGCAGCTCGGTCAGATC |
| TUR | 6,999,519 | 6,999,539 | GCTCCATAGGTCACGAACATC |
| TDF | 6,997,313 | 6,997,334 | GCAACTTCTTCAGCCTTCTTCC |
| TDP | 6,997,286 | 6,997,312 | CCTCCATTCTTCACATCGCACCTAGGA |
| TDR | 6,997,244 | 6,997,267 | GTGCTGGATTTGGATCTGTGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTATATCAGGCCCACTGC |
| SD | AAGAGTGGGCGTAGCTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14012D-G9 | VGB6 | C57BL/6NTac | |
| 14012D-C11 | VGB6 | C57BL/6NTac | |
| 14012D-G11 | VGB6 | C57BL/6NTac | |
| 14012D-G6 | VGB6 | C57BL/6NTac | |
