| Gene | 1700039E15Rik |
| Chromosome | 7 |
| Deletion Start | 45,282,876 |
| Deletion End | 45,288,559 |
| Deletion Size | 5,684 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,282,883 | 45,282,901 | TGGCGACTTCGTTGCTGAG |
| TUP | 45,282,904 | 45,282,929 | CATTCGTCATCTTCCTCGTGATGGGC |
| TUR | 45,282,938 | 45,282,956 | CATAGTGCCGCTGGTTGTG |
| TDF | 45,288,238 | 45,288,256 | CAGTCACGGTGCCCAACAG |
| TDP | 45,288,257 | 45,288,279 | AGTCACCCAGTGGCCTGTGCACC |
| TDR | 45,288,287 | 45,288,309 | CCTGATTTCGGAGGGTGAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGACCATAGAGGTAAGGCCG |
| SD | GGAGGTCAGCCTCACTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14013A-C2 | VGB6 | C57BL/6NTac | |
| 14013A-C5 | VGB6 | C57BL/6NTac | |
| 14013A-D3 | VGB6 | C57BL/6NTac | |
| 14013A-F7 | VGB6 | C57BL/6NTac | |
| 14013A-F11 | VGB6 | C57BL/6NTac | |
| 14013A-H1 | VGB6 | C57BL/6NTac | |
