14014 1700040I03Rik Status: ES cell clone microinjected
| Gene | 1700040I03Rik |
| Chromosome | 6 |
| Deletion Start | 85,401,268 |
| Deletion End | 85,397,174 |
| Deletion Size | 4,095 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,400,962 | 85,400,980 | AGCGGGTGGATGCTGGTTC |
| TUP | 85,400,937 | 85,400,960 | CGGGTCAGTGAGTAGGCACAACCT |
| TUR | 85,400,883 | 85,400,904 | TCAGTGGGAGGAAAGCTGAAAG |
| TDF | 85,397,325 | 85,397,345 | GTGTTGGGTGCTGAGAAAGTG |
| TDP | 85,397,298 | 85,397,323 | CCAACGCTATACTGCTCTTCATGCCC |
| TDR | 85,397,270 | 85,397,291 | CAGAGAACGGCGGATCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | GCGTCTATAAGGAGGAGTGC |
| SD | GCAGATGAGCTCCCTACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14014A-G9 | VGB6 | C57BL/6NTac | |
| 14014A-C5 | VGB6 | C57BL/6NTac | |
| 14014A-C10 | VGB6 | C57BL/6NTac | |
| 14014A-G6 | VGB6 | C57BL/6NTac | |
| 14014A-E6 | VGB6 | C57BL/6NTac | |
| 14014A-F2 | VGB6 | C57BL/6NTac | |
