14017 2010001M09Rik Status: Cancelled
| Gene | 2010001M09Rik |
| Chromosome | 18 |
| Deletion Start | 35,649,330 |
| Deletion End | 35,647,570 |
| Deletion Size | 1,761 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,649,223 | 35,649,244 | TCCCACACTGGATGATGAAGAG |
| TUP | 35,649,197 | 35,649,219 | TACTCGGCTCATATGCCGGCTCA |
| TUR | 35,649,146 | 35,649,166 | CAGGCAGAGCTTACCTGGAAG |
| TDF | 35,647,770 | 35,647,789 | CCGCTTTGCTCCTCGACTTC |
| TDP | 35,647,747 | 35,647,769 | CCCAAGGGCGGGTTAGAGCTCAA |
| TDR | 35,647,689 | 35,647,712 | CAAACTCACCCAGGTAGTGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCCTCCTGGAGACTGTGACC |
| SD | GGTACCAGGGCAGTTCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
