| Gene | Zfp846 |
| Chromosome | 9 |
| Deletion Start | 20,587,894 |
| Deletion End | 20,594,544 |
| Deletion Size | 6,651 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,588,262 | 20,588,282 | GAAAGAAGGGCCTCTGGTCTG |
| TUP | 20,588,284 | 20,588,305 | TCGTGGCAAGGTCTGCTCAGCT |
| TUR | 20,588,325 | 20,588,347 | GTCAGTCTGCTCCACTCATTTAC |
| TDF | 20,594,301 | 20,594,323 | AGCCGAACTGAACATATCCGAAC |
| TDP | 20,594,324 | 20,594,347 | TCACACTGGTGAGAGGCCCTATGA |
| TDR | 20,594,378 | 20,594,401 | ACGTGGTGAATGAGGTTAGATGAG |
PCR Primers
| Primer | Sequence |
| SU | CACGGATTTCACATTAGGGC |
| SD | AACAGGCCTTATTGCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14020A-A3 | VGB6 | C57BL/6NTac | |
| 14020A-B12 | VGB6 | C57BL/6NTac | |
| 14020A-C5 | VGB6 | C57BL/6NTac | |
| 14020A-G11 | VGB6 | C57BL/6NTac | |
