14021   2310003F16Rik     Status: ES cell colonies screened / QC no positives

Gene2310003F16Rik
Chromosome2
Deletion Start121,457,109
Deletion End121,458,263
Deletion Size1,155
Genome Build38
MGI IDMGI:1914943
NCBI Gene ID67693
BAC ID388C12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,457,192121,457,211TCCCGAGAAGCCAAGGAAAC
TUP121,457,213121,457,236TGACAGTGGTGCTGCTGACCTAGA
TUR121,457,240121,457,260TCCTCCGCATAGTCAGTAACC
TDF121,457,911121,457,931TTCAGTGAGTAGCTGGGAGTG
TDP121,457,933121,457,959TCCTTCTGGGAGTTTATAGCAGAGGCA
TDR121,457,995121,458,013GACCTTCGCCAGTTCCTTC

PCR Primers

PrimerSequence
SUTTCCTATTCGGGATCATTCG
SDATCTGCTGCTTAGAGCTGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR103 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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